Related Experiment Video

Updated: Mar 19, 2026

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

11.7K

GON4L Drives Cancer Growth through a YY1-Androgen Receptor-CD24 Axis

Neeraj Agarwal1, Garrett M Dancik2, Andrew Goodspeed3

  • 1Department of Pharmacology, University of Colorado, Denver, Colorado. Department of Surgery (Urology), University of Colorado, Denver, Colorado.

Cancer Research
|June 18, 2016
PubMed

Insights

Researchers identified GON4L as a key driver of cancer growth by regulating CD24 expression. Inhibiting GON4L suppressed tumor growth in bladder, lung, prostate, and breast cancers, offering new therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • CD24 is a biomarker for poor prognosis and promotes tumor growth and metastasis.
  • Gain-of-function genes identified in large patient cohorts present therapeutic targets.

Purpose of the Study:

  • To identify regulators of CD24 expression and assess their role in cancer growth.
  • To investigate the therapeutic potential of targeting identified regulators.

Main Methods:

  • Screening of a pooled shRNA library against 176 candidate gain-of-function genes.
  • Depletion of GON4L in human bladder cancer cells and assessment of proliferation and tumor xenograft growth.
  • Analysis of GON4L, YY1, and CD24 expression in clinical cancer specimens and other cancer cell lines.

Main Results:

  • GON4L was identified as a regulator of CD24 expression.
  • GON4L depletion reduced CD24 levels, inhibited cell proliferation, and suppressed tumor xenograft growth.
  • Elevated expression of GON4L, YY1, and CD24 was observed in bladder cancer tissues.
  • Silencing GON4L and YY1 suppressed CD24 expression and growth in lung, prostate, and breast cancer cells.

Conclusions:

  • GON4L is a novel driver of cancer growth, interacting with YY1 and the androgen receptor to promote CD24 expression.
  • The GON4L-YY1-androgen receptor-CD24 pathway is crucial for cancer progression and offers potential therapeutic targets.
  • GON4L represents a promising biomarker and therapeutic target across multiple cancer types.

More Related Videos

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures07:48

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

11.9K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

1.6K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.3K
Tumor Progression02:07

Tumor Progression

3.5K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K