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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

Reducing the heterogeneity of xylan through processing.

Wei Zhang¹, Amanda M Johnson², Justin R Barone³

¹Macromolecules and Interfaces Institute, Virginia Tech, Blacksburg, VA, 24061, United States; Department of Sustainable Biomaterials, Virginia Tech, Blacksburg, VA, 24061, United States.

Carbohydrate Polymers

|June 18, 2016

Summary

This summary is machine-generated.

Glycerol thermal processing (GTP) effectively fractionates hardwood xylan, yielding high molecular weight, purified xylan (WIX) suitable for advanced applications. This method preserves glucuronoxylan integrity during biomass pretreatment.

Keywords:

Fractionation Glucuronoxylan Glycerol thermal processing Molecular weight analysis Rhamnan Water insoluble xylan

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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

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Published on: October 16, 2017

Area of Science:

Biomass Fractionation
Biopolymer Chemistry
Sustainable Materials

Background:

Hemicellulose, particularly glucuronoxylan, is a major component of hardwood biomass.
Efficient fractionation of xylan is crucial for its utilization in value-added applications.
Existing methods often lead to xylan degradation during biomass processing.

Purpose of the Study:

To investigate the effectiveness of glycerol thermal processing (GTP) for hardwood biomass fractionation.
To selectively isolate and characterize glucuronoxylan fractions.
To evaluate the impact of GTP severity on xylan recovery and purity.

Main Methods:

Hardwood biomass was subjected to glycerol thermal processing (GTP) at 200–240°C.
Sequential extraction using water, organic solvents, and mild alkali was performed.
Isolated xylan fractions (WIX and WSX) were characterized by molecular weight, purity, and structural analysis.

Main Results:

GTP pretreatment preserved up to 80% of initial xylan, limiting degradation.
Mild alkali extraction yielded two distinct xylan fractions: water-insoluble xylan (WIX) and water-soluble xylan (WSX).
WIX exhibited high molecular weight (DP > 100) and purity (up to 84%), with narrow polydispersity (1.1–1.25), while WSX had lower DP and higher substitution.

Conclusions:

GTP is a viable method for stepwise biopolymer fractionation of hardwood biomass.
The process allows for the preferential isolation of high molecular weight glucuronoxylan (WIX).
Isolated WIX shows potential for applications in healthcare materials and advanced packaging.