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The in vitro selection world.

Kenan Jijakli1, Basel Khraiwesh1, Weiqi Fu1

  • 1Laboratory of Algal, Systems, and Synthetic Biology, Division of Science and Math, and Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi, P.O. Box 129188, Abu Dhabi, United Arab Emirates.

Methods (San Diego, Calif.)
|June 18, 2016
PubMed
Summary
This summary is machine-generated.

In vitro selection isolates desired molecules from vast libraries. Recent advancements expand its use to proteins and integrate sequencing and computational tools for better analysis.

Keywords:
AptamerIn silicoIn vitro selectionIn vivoNext generation sequencingPhage displayRNA ligandsSELEX

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biochemistry

Background:

  • In vitro selection is a powerful method for isolating functional molecules from large combinatorial libraries.
  • The technique has been instrumental in scientific discoveries for over 25 years.

Purpose of the Study:

  • To provide a comprehensive overview of in vitro selection, including its history and recent advancements.
  • To highlight the expansion of in vitro selection beyond nucleic acids to polypeptides and proteins.
  • To discuss the integration of next-generation sequencing and computational tools with in vitro selection.

Main Methods:

  • Iterative cycles of selection, amplification, and mutagenesis.
  • Application of in vitro selection to nucleic acids, polypeptides, and proteins.
  • Integration with next-generation sequencing and computational analysis.

Main Results:

  • In vitro selection enables the isolation of molecules with specific properties from libraries up to 10^16 in size.
  • Recent developments have enhanced the capabilities and broadened the scope of in vitro selection.
  • Next-generation sequencing and computational tools significantly improve data analysis from in vitro selection experiments.

Conclusions:

  • In vitro selection remains a vital technique for molecular discovery and engineering.
  • The synergy between in vitro selection, sequencing, and computational tools offers unprecedented power for biological research.
  • Understanding the interplay between in vitro and in vivo selection methods is crucial for their optimal application.