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Related Experiment Video

Updated: Mar 19, 2026

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Published on: August 17, 2012

Gene expression analysis at multiple time-points identifies key genes for nerve regeneration.

Bin Pan¹, Yi Liu¹, Jia-Yin Yan¹

¹Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.

|June 18, 2016

Summary

This study details gene expression changes during peripheral nerve injury and regeneration. It identifies key pathways and biological processes crucial for axon regrowth after sciatic nerve crush.

Keywords:

Schwann cell Wallerian degeneration mRNA microarray peripheral nerve injury regeneration

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Last Updated: Mar 19, 2026

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The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

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Published on: August 11, 2016

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

Peripheral nerve injury triggers complex cellular responses.
Understanding gene expression is vital for elucidating regeneration mechanisms.

Purpose of the Study:

To comprehensively understand gene expression during Wallerian degeneration and axon regeneration.
To identify key molecular pathways involved in peripheral nerve repair.

Main Methods:

Microarray analysis to detect gene expression changes at 0, 3, 7, and 14 days post-sciatic nerve crush.
Bioinformatic analysis to predict functions of differentially expressed messenger RNAs (mRNAs).
Quantitative real-time polymerase chain reaction (qRT-PCR) for validation of microarray results and key pathways.

Main Results:

Identified differentially expressed mRNAs at 3, 7, and 14 days post-injury compared to controls.
Discovered nine general trends in gene expression changes.
Verified key signaling pathways and nine biological processes associated with nerve regeneration.

Conclusions:

The identified differentially expressed genes, biological processes, and pathways offer insights into molecular mechanisms of peripheral nerve regeneration.
This research contributes to understanding the molecular basis of axonal regeneration.