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Whole-Genome Sequencing Analysis of Methicillin-Resistant Staphylococcus simulans Causing Surgical Site Infection

Jian Chen1, Qiang Fang1

  • 1Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China cjicu88@126.com.

Genome Announcements
|June 18, 2016
PubMed

Insights

Staphylococcus simulans, usually harmless, can cause infections. Sequencing its genome revealed adhesion proteins that may explain its ability to infect humans, particularly in surgical sites.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Staphylococcus simulans is a commensal bacterium found in human and animal microbiota.
  • It is infrequently linked to invasive human infections.
  • This study focuses on a strain causing a surgical site infection.

Purpose of the Study:

  • To present the genome sequence of Staphylococcus simulans CJ16.
  • To identify potential virulence factors contributing to infection.

Main Methods:

  • Whole genome sequencing of Staphylococcus simulans CJ16.
  • Bioinformatic analysis to identify genes encoding adhesion proteins.

Main Results:

  • The genome sequence of S. simulans CJ16 was determined.
  • Genes for adhesion proteins, including fibronectin-binding protein (FnbA), elastin-binding protein (EbpS), and cell wall-anchored proteins (SasA, SasF, SasH), were identified.
  • These proteins may facilitate bacterial survival and infection.

Conclusions:

  • The genome of S. simulans CJ16 provides insights into its pathogenic potential.
  • Adhesion proteins identified in the genome may play a role in S. simulans-associated surgical site infections.

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