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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Published on: May 1, 2019
15.7K
Stress Beyond Translation: Poxviruses and More
1Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas. JWLiem@uams.edu.
Viruses
|June 18, 2016
Summary
Poxviruses manipulate host translation, forming antiviral granules (AVGs) similar to stress granules (SGs). These AVGs may be linked to viral sensing and reprogramming cellular functions during infection.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Poxviruses are large DNA viruses replicating in the cytoplasm.
- They rely on host translation machinery for protein synthesis.
- Poxviruses actively reprogram host translation regulation.
Purpose of the Study:
- To review how poxviruses manipulate host translation.
- To compare antiviral granules (AVGs) with stress granules (SGs).
- To discuss cytoplasmic sensing bodies during viral infections.
Main Methods:
- Review of recent findings on poxvirus-host translation interactions.
- Comparative analysis of AVGs and SGs.
- Examination of RNA- and DNA-sensing bodies.
Main Results:
- AVGs form around poxvirus factories and contain SG proteins.
- AVG formation is linked to abortive infections.
- Cytoplasmic complexes can contain sensing molecules.
Conclusions:
- AVGs share similarities with stress granules and viral sensing bodies.
- Understanding AVGs offers insights into viral infection mechanisms.
- Further research into AVGs and sensing bodies is warranted.
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