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Protein tyrosine phosphatase PTPRB regulates Src phosphorylation and tumour progression in NSCLC

Yinliang Qi1,2, Yuanchang Dai2, Shuyu Gui1

  • 1Department of Respiratory Disease, Anhui Medical University, Hefei, Anhui, China.

Clinical and Experimental Pharmacology & Physiology
|June 18, 2016
PubMed

Insights

Protein tyrosine phosphatase receptor type B (PTPRB) suppresses non-small-cell lung cancer (NSCLC) growth and invasion. Down-regulation of PTPRB correlates with poor patient survival, suggesting its potential as a prognostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein tyrosine-phosphatases (PTPs) are crucial in cellular signaling and implicated in cancer.
  • The specific role of protein tyrosine phosphatase receptor type B (PTPRB) in non-small-cell lung cancer (NSCLC) tumorigenesis remains uncharacterized.

Purpose of the Study:

  • To investigate the function of PTPRB in NSCLC development and progression.
  • To determine if PTPRB expression is associated with patient prognosis in NSCLC.

Main Methods:

  • Utilized lentiviral vectors to overexpress or knockdown PTPRB in NSCLC cell lines (A549, H1299).
  • Assessed cell proliferation, colony formation, soft agar growth, and invasion assays in vitro.
  • Evaluated PTPRB's in vivo function using an A549 xenograft mouse model.
  • Quantified PTPRB expression in NSCLC patient samples via quantitative PCR (qPCR).
  • Performed Kaplan-Meier and multivariate analyses to correlate PTPRB expression with overall survival (OS).

Main Results:

  • PTPRB overexpression inhibited NSCLC cell proliferation, colony formation, soft agar growth, and invasion.
  • PTPRB knockdown enhanced Src phosphorylation and cell invasion, effects reversible by a Src inhibitor (PP2).
  • PTPRB was found to be downregulated in NSCLC patient samples.
  • Lower PTPRB expression was significantly associated with poorer patient overall survival.

Conclusions:

  • PTPRB plays a tumor-suppressive role in NSCLC by regulating Src phosphorylation and inhibiting tumorigenesis.
  • PTPRB expression level serves as an independent prognostic biomarker for NSCLC patients.
Keywords:
NSCLCPTPRBprognostic biomarkertumour progression

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