Related Experiment Video
Updated: Mar 19, 2026

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
Published on: February 6, 2015
Discovery bioanalysis and in vivo pharmacology as an integrated process: a case study in oncology drug discovery
Antonio Grondin1, Arnaud Pillon1, Isabelle Vandenberghe1
1Laboratoires Pierre Fabre, Institut de Recherche Pierre Fabre, 3, Avenue Hubert Curien, BP 13562, 31035 Toulouse, France.
Background:
A bioanalytical team dedicated to in vivo pharmacology was set up to accelerate the selection and characterization of compounds to be evaluated in animal models in oncology.
Results:
A DBS-based serial microsampling procedure was optimized from sample collection to extraction to obtain a generic procedure. UHPLC-high-resolution mass spectrometer configuration allowed for fast quantitative and qualitative analysis. Using an optimized lead compound, we show how bioanalysis supported in vivo pharmacology by generating blood and tumor exposure, drug monitoring and PK/PD data.
Conclusion:
This process provided unique opportunities for the characterization of drug properties, selection and assessment of compounds in animal models and to support and expedite proof-of-concept studies in oncology.
More Related Videos
13:17In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology
Published on: September 29, 2023
07:40Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
Related Concept Videos
Drug Discovery: Overview
Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems
Preclinical Development: Overview
Biopharmaceutics and Pharmacokinetics: Overview
Analysis of Population Pharmacokinetic Data
Measurement of Bioavailability: Pharmacodynamic Methods