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A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

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Growth Factor and Interleukin Concentrations in Amniotic Membrane-Conditioned Medium.

Mona Bischoff^1,2, Tanja Stachon¹, Berthold Seitz^1,2

¹a Department of Ophthalmology , Saarland University Medical Center , Homburg/Saar , Germany.

Current Eye Research

|June 18, 2016

Summary

Amniotic membrane-conditioned medium (AMM) eye drops show potential for treating corneal defects. Different preparation methods impact concentrations of epidermal growth factor (EGF), fibroblast growth factor basic (FGFb), IL-6, and IL-8, requiring further optimization.

Keywords:

Amniotic membrane conditioned medium erosion growth factor interleukin therapy-resistant corneal epithelial defects

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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Area of Science:

Ophthalmology
Regenerative Medicine
Biochemistry

Background:

Therapy-resistant corneal epithelial defects pose a significant clinical challenge.
Amniotic membrane-conditioned medium (AMM) is being explored as a novel therapeutic agent.
AMM contains various growth factors and cytokines with potential regenerative properties.

Purpose of the Study:

To quantify the concentrations of key growth factors and interleukins in AMM.
To investigate the impact of different AMM preparation methods on these concentrations.
To assess the potential of AMM for treating corneal epithelial defects.

Main Methods:

Amniotic membranes from 10 placentas were processed and stored.
Membrane pieces were incubated in DMEM culture medium for varying durations (8 hours to 4 weeks).
Enzyme-linked immunosorbent assay (ELISA) was used to measure concentrations of EGF, NGF, VEGF, TGFβ1, FGFb, IL-1β, IL-6, and IL-8.

Main Results:

NGF, VEGF, TGFβ1, and IL-1β were below detection limits.
Detectable concentrations of EGF, FGFb, IL-6, and IL-8 were found.
Significant differences in IL-6 and IL-8 concentrations were observed between preparation methods at specific time points.

Conclusions:

AMM contains EGF, FGFb, IL-6, and IL-8, suggesting therapeutic potential for corneal defects.
Preparation methods significantly influence IL-6 and IL-8 levels.
Further research is needed to determine optimal preparation and harvesting times for AMM therapy.