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Related Experiment Videos

DHPR activity decrease in dried blood spots stored at 4 degrees C.

G Lenzi1, D Pistone, E Zammarchi

  • 1Department of Paediatrics, University of Florence, Italy.

Enzyme
|January 1, 1989
PubMed
Summary

Dihydropteridine reductase (DHPR) activity in dried blood spots is measurable using the Arai method. DHPR activity on stored blood spots significantly decreases after one year, impacting diagnostic reliability.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Pediatrics

Background:

  • Dihydropteridine reductase (DHPR) is crucial for phenylalanine metabolism.
  • Accurate measurement of DHPR activity is essential for diagnosing metabolic disorders.
  • Dried blood spots are a common medium for neonatal screening.

Purpose of the Study:

  • To measure DHPR activity in various subject groups using dried blood spots.
  • To assess the stability of DHPR enzymatic activity in stored dried blood spots.
  • To evaluate the reliability of the Arai method for DHPR activity measurement.

Main Methods:

  • Enzymatic activity of DHPR was measured using the Arai method.
  • Blood samples were collected from 286 subjects, including newborns, hyperphenylalaninemia patients, normal individuals, and DHPR-deficient patients.

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  • Dried blood spots were stored at 4°C for stability assessment over one year.

    • Main Results:

    • DHPR activity was successfully measured in all subject groups.
    • A significant decrease in DHPR activity (to one-third of original value) was observed after one year of storage at 4°C.
    • The reduced activity after storage approached the mean value of controls minus 2 standard deviations.

    Conclusions:

    • The Arai method is suitable for measuring DHPR activity in dried blood spots.
    • Long-term storage of dried blood spots at 4°C leads to substantial loss of DHPR enzymatic activity.
    • Storage conditions must be carefully considered to ensure the accuracy of DHPR activity measurements for diagnostic purposes.