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Related Experiment Video

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Tuneable nanoparticle-nanofiber composite substrate for improved cellular adhesion.

Ariana M Nicolini1, Tyler D Toth2, Jeong-Yeol Yoon3

  • 1Biomedical Engineering Graduate Interdisciplinary Program, The University of Arizona, Tucson, AZ 85721, USA.

Colloids and Surfaces. B, Biointerfaces
|June 18, 2016
PubMed
Summary

This study introduces a new reverse potential electrospinning method to create nanoparticle-embedded fibers. This technique enhances cellular adhesion for tissue engineering scaffolds and biomaterials.

Keywords:
Functionalized nanoparticlesRGD ligandReverse potential electrospinningSurface tensionSurfactant

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Tissue Engineering

Background:

  • Electrospinning is a common method for creating nanofibers.
  • Tailoring fiber properties is crucial for biomaterial applications.
  • Improving cellular adhesion is key for tissue regeneration.

Purpose of the Study:

  • To develop a novel reverse potential electrospinning technique.
  • To fabricate nanoparticle-embedded composite fibers with tunable properties.
  • To evaluate the effect of these fibers on cellular adhesion.

Main Methods:

  • Electrospinning of polycaprolactone (PCL) nanofibers using traditional positive (PP) and reverse potential (RP) modes.
  • Incorporation of 300nm polystyrene (PS) fluorescent particles with carboxyl and amine groups.
  • Culturing human umbilical vein endothelial cells (HUVECs) on fabricated substrates.
  • Assessing cellular viability and adhesion over 5 days.

Main Results:

  • Reverse potential electrospinning significantly decreased fiber diameter.
  • Nanoparticle incorporation in RP mode reduced fiber diameter to 440±80nm.
  • Cell adhesion improved by 180% with carboxylated particles and 480% with RGD ligand functionalization.

Conclusions:

  • Reverse potential electrospinning offers enhanced control over fiber morphology.
  • Nanoparticle-embedded fibers show improved cellular adhesion properties.
  • This technique is promising for developing advanced biomaterials and tissue scaffolds.