Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Type 2 and type 1 diabetes have opposing effects on the systemic murine complement alternative pathway.

iScience·2026

Same author

Loss of PTEN as an Independent Poor Prognosis Indicator in Lung Adenocarcinoma, but Not in Squamous Cell Carcinoma, Is Associated with an Immunosuppressive Tumor Microenvironment and Distinct Co-Mutational Profiles.

Medical sciences (Basel, Switzerland)·2026

Same author

Foundation models and deep learning for cancer drug response prediction: a framework for data, metrics, and validation.

Briefings in bioinformatics·2026

Same author

Tumor-Derived Complement C3 Overexpression in STK11-Mutant Lung Adenocarcinoma Drives Tumor Growth and Immune Checkpoint Inhibitor Resistance.

Cancer immunology research·2026

Same author

Single-cell and spatial transcriptomic profiling of cardiac fibroblasts following myocardial infarction.

Scientific data·2026

Same author

Insights and perspectives into the discovery of complement-related biomarkers in cancer.

Current opinion in immunology·2025

Search research articles

Related Experiment Video

Updated: Mar 19, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

EventPointer: an effective identification of alternative splicing events using junction arrays.

Juan P Romero^1,2, Ander Muniategui^1,2, Fernando J De Miguel³

¹CEIT, Parque Tecnológico de San Sebastián, Paseo Mikeletegi 48, 20009, San Sebastián, Gipuzkoa, Spain.

|June 19, 2016

Summary

We developed EventPointer, a new R package for analyzing alternative splicing (AS) events using HTA 2.0 arrays. This tool offers a powerful and efficient alternative to RNA-seq for detecting AS with high accuracy and low false discovery rates.

Keywords:

Alternative splicing Junction microarrays Protein domains

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Related Experiment Videos

Last Updated: Mar 19, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Area of Science:

Transcriptomics
Molecular Biology
Bioinformatics

Background:

Alternative splicing (AS) generates transcriptome diversity in eukaryotes, with growing interest in its role in pathologies.
Traditional AS detection methods using arrays had high false discovery rates (FDR).
Current Human Transcriptome Array 2.0 (HTA 2.0) analysis software has limitations in exploiting array potential and study designs.

Purpose of the Study:

To develop a novel statistical algorithm and software to effectively detect and analyze AS events using HTA 2.0 arrays.
To overcome the limitations of existing software for HTA 2.0 array analysis.
To provide a robust tool for AS event detection applicable to complex experimental designs.

Main Methods:

Developed EventPointer, an R package utilizing the aroma.affymetrix framework.
Employed a linear model to analyze AS events from HTA 2.0 array data.
Integrated analysis of exons and junctions for comprehensive event detection.

Main Results:

EventPointer successfully identifies AS event types, fold changes, statistical significance, and affected protein domains.
Demonstrated extremely low FDR, with only one false positive among the top 200 tested events.
The software is publicly available on GitHub.

Conclusions:

EventPointer enhances HTA 2.0 array capabilities for AS event detection, serving as an alternative to RNA-seq.
The software simplifies and accelerates AS analysis while reducing computational demands.
Enables robust AS event detection in complex experimental designs with high accuracy.