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Related Experiment Video

Updated: Mar 19, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

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Published on: April 23, 2019

High-Throughput Screening Using Mass Spectrometry within Drug Discovery.

Mattias Rohman¹, Jonathan Wingfield²

¹Reagent and Assay Development, AstraZeneca R&D, Mölndal, Sweden. mattias.rohman@astrazeneca.com.

Methods in Molecular Biology (Clifton, N.J.)

|June 19, 2016

Summary

RapidFire™ coupled to mass spectrometry (MS) offers high-throughput screening by using solid-phase extraction to remove salts. This method significantly reduces sample processing time for faster MS analysis.

Keywords:

Electrospray (ESI)High-throughput screening Ionization Mass charge (m/z)Mass spectrometry RapidFire™Triple-quadrupole mass spectrometry

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

Area of Science:

Analytical Chemistry
Biochemistry

Background:

Mass spectrometry (MS) requires analyte ionization, often achieved through electrospray ionization (ESI).
High salt concentrations in samples can suppress the MS signal by interfering with analyte ionization.
Traditional methods using liquid chromatography (LC) for salt removal are time-consuming.

Purpose of the Study:

To introduce a high-throughput screening method using RapidFire™ coupled to a triple-quadrupole MS.
To demonstrate the effectiveness of solid-phase extraction for rapid de-salting of samples for MS analysis.

Main Methods:

Utilized RapidFire™ technology for sample preparation.
Employed solid-phase extraction (SPE) to remove salts from biological samples.
Coupled the RapidFire™ system to a triple-quadrupole mass spectrometer for analysis.

Main Results:

Achieved rapid de-salting of samples via solid-phase extraction.
Reduced sample processing time significantly compared to traditional LC methods.
Enabled sample injection into the MS approximately every 10 seconds, facilitating high-throughput screening.

Conclusions:

The RapidFire™-MS system provides an efficient solution for high-throughput screening by overcoming salt-induced signal suppression.
Solid-phase extraction integrated with RapidFire™ is a viable strategy for accelerating MS-based assays.
This approach enhances analytical throughput for biochemical analyses.