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Updated: Mar 19, 2026

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Published on: September 22, 2019

High-Throughput Cell Toxicity Assays.

David Murray¹, Lisa McWilliams², Mark Wigglesworth²

¹Discovery Sciences, Innovative Medicines, AstraZeneca, Alderley Park, Macclesfield, SK10 4TG, UK. david.murray@astrazeneca.com.

Methods in Molecular Biology (Clifton, N.J.)

|June 19, 2016

Summary

This summary is machine-generated.

High-throughput screening (HTS) is crucial for drug discovery. This study details methods for large-scale cell toxicity screening in HTS labs to identify safe drug candidates.

Keywords:

Apoptosis Cell-based assays HTS High-throughput screening Phenotypic Resazurin Toxicity

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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Area of Science:

Drug Discovery
Toxicology
Cell Biology

Background:

Compound-driven cell toxicity is critical in drug discovery.
High-throughput screening (HTS) identifies potential drug compounds.
Phenotypic drug discovery relies on cell-based assays, increasing the need for toxicity screening.

Purpose of the Study:

To describe methods for large-scale cell toxicity screening.
To ensure false hits are not progressed in drug discovery.
To prevent toxic liabilities in lead compounds.

Main Methods:

Development of methods for very large-scale cell toxicity screening.
Implementation of these methods in the AstraZeneca HTS laboratory.
Integration of toxicity assessment within HTS workflows.

Main Results:

Establishment of efficient, large-scale cell toxicity screening capabilities.
Improved identification of non-toxic hit compounds.
Reduced risk of late-stage project failure due to compound toxicity.

Conclusions:

Large-scale cell toxicity screening is essential for modern drug discovery.
The described methods enable efficient toxicity profiling of HTS compounds.
Accurate toxicity assessment improves the success rate of drug development.