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Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

Published on: May 10, 2020

Cardiac Myocyte Exosome Isolation.

Zulfiqar A Malik^1,2, Tingting T Liu¹, Anne A Knowlton^3,4,5

¹Molecular & Cellular Cardiology, Division of Cardiovascular Medicine, University of California, Davis, Shields Avenue, Davis, CA, 95616, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 19, 2016

Summary

Researchers developed a simple, cost-effective method for isolating and analyzing cardiac myocyte exosomes. This advance addresses challenges in exosome purification and analysis, crucial for understanding cellular communication.

Keywords:

Acetyl choline esterase Cardiac myocytes Cell signaling Exosomes Heart Protocol

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Last Updated: Mar 19, 2026

Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

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Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

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Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

Area of Science:

Cell Biology
Extracellular Vesicles
Biochemistry

Background:

Exosomes are small extracellular vesicles involved in intercellular communication.
They shuttle proteins, mRNA, and microRNAs between cells.
Current exosome isolation and analysis methods face challenges and lack standardization.

Purpose of the Study:

To develop a simple and cost-effective method for isolating cardiac myocyte exosomes.
To address the urgent need for reliable exosome purification and analysis protocols.

Main Methods:

Isolation of exosomes from conditioned cell media.
Analysis of cardiac myocyte exosomes.

Main Results:

A simple, cost-effective protocol for exosome isolation and analysis was established.
The study focused on cardiac myocyte exosomes.

Conclusions:

The developed method provides a reliable approach for exosome purification and analysis.
This contributes to advancing research in extracellular vesicle biology and cardiac function.