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Distinctive features of procoagulant response of monocytes from diabetic patients
1Laboratoire d'Hématologie, Centre Hospitalier Régional, Lille, France.
Abstract:
The procoagulant activity (PCA) of disrupted monocytes was examined in 32 diabetic patients (26 with insulin-dependent and 6 with non-insulin-dependent diabetes) versus 30 control subjects. Diabetes monocytes exhibited a weak PCA before any incubation, associated in 10 cases with a significant amount of factor VII activity. Incubation led to a significant rise in PCA in diabetes cells, when stimulated with lipopolysaccharide or not, and in control cells only after stimulation. In incubated diabetes cells, PCA was prothrombinase-like when factor VII was associated with the freshly isolated cells, and tissue factor-like (as in the controls) when no factor VII was associated with the cells. The characteristics of PCA were not correlated with clinical features or with the type of diabetes. Our study suggests that diabetes monocytes exhibit a higher level of PCA than control ones, possibly corresponding to an in vivo stimulation, or at least a higher responsiveness to stimuli occurring in vitro.
Insights
Diabetic patients
Area of Science:
- Hematology
- Endocrinology
- Immunology
Background:
- Monocytes play a crucial role in hemostasis and inflammation.
- Diabetes mellitus is associated with an increased risk of thrombotic events.
- The procoagulant activity (PCA) of monocytes in diabetes is not fully understood.
Purpose of the Study:
- To investigate the procoagulant activity (PCA) of monocytes in diabetic patients compared to healthy controls.
- To characterize the nature of PCA in diabetic monocytes.
- To explore potential correlations between PCA and clinical features of diabetes.
Main Methods:
- Disrupted monocytes were isolated from 32 diabetic patients and 30 control subjects.
- Procoagulant activity was measured before and after incubation, with and without lipopolysaccharide stimulation.
- Factor VII and tissue factor-like activities were assessed.
Main Results:
- Diabetes monocytes showed weak baseline PCA, sometimes associated with factor VII activity.
- Incubation significantly increased PCA in diabetic monocytes, irrespective of stimulation.
- Control monocytes' PCA increased only after stimulation.
- PCA characteristics in diabetic monocytes varied based on associated factor VII levels.
Conclusions:
- Monocytes from diabetic patients exhibit enhanced procoagulant activity compared to controls.
- This heightened PCA may result from in vivo stimulation or increased in vitro responsiveness.
- The findings suggest a potential contribution of monocyte PCA to the thrombotic risk in diabetes.