[Progress of chiral countercurrent chromatography].

Area of Science:

• Analytical Chemistry
• Separation Science
• Chiral Separations

Background:

• Chiral countercurrent chromatography (CCC) is an advanced liquid-liquid separation technique.
• The demand for enantiomerically pure compounds in pharmaceuticals and other industries drives the need for efficient chiral separation methods.

Purpose of the Study:

• To summarize the key characteristics of chiral countercurrent chromatography.
• To review the progress and applications of chiral CCC.
• To discuss various chiral selectors employed in high-speed countercurrent chromatography (HSCCC).

Main Methods:

• Review of literature on chiral countercurrent chromatography.
• Identification and categorization of five core characteristics of chiral CCC.
• Analysis of advancements in HSCCC techniques.
• Compilation of commonly used chiral selectors.

Main Results:

• Five essential characteristics of chiral CCC have been identified.
• Significant progress in the field of chiral CCC has been observed.
• Various chiral selectors, including amino acid derivatives, cyclodextrin derivatives, chiral organic acids, polysaccharides, and bovine serum albumin (BSA), are effective in HSCCC.

Conclusions:

• Chiral CCC is a versatile and powerful technique for enantiomeric separation.
• The selection of appropriate chiral selectors is crucial for successful chiral separations using HSCCC.
• Continued development in CCC technology promises further advancements in chiral analysis.