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Transgenic Organisms

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Related Experiment Video

Updated: Mar 19, 2026

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Published on: May 5, 2014

Human HLA-Ev (147) Expression in Transgenic Animals.

R Matsuura¹, A Maeda¹, R Sakai¹

¹Department of Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; and Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Transplantation Proceedings

|June 21, 2016

Summary

This summary is machine-generated.

We optimized a synthetic HLA-Ev (147) gene for xenotransplantation and created transgenic mice. This engineered gene effectively regulates immune responses in pig endothelial cells and shows broad expression in mouse organs.

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Area of Science:

Immunology
Genetics
Transplantation Biology

Background:

Previous work established S147C for HLA-E substitution in xenotransplantation.
This study focuses on codon exchange for HLA-Ev (147) to enhance its utility.

Purpose of the Study:

To establish a functional, codon-optimized HLA-Ev (147) gene for xenotransplantation.
To generate and characterize transgenic mice expressing the modified gene.

Main Methods:

Constructed a new plasmid with codon-exchanged HLA-Ev (147), IRES, and human beta 2-microglobulin in a pCXN2 vector.
Assessed natural killer cell and macrophage cytotoxicity in pig endothelial cells transfected with the construct.
Produced transgenic mice via microinjection and confirmed gene expression in various organs.

Main Results:

Successfully transfected pig endothelial cells, confirming molecule expression.
Demonstrated that the expressed molecules effectively regulated natural killer cell and macrophage cytotoxicity.
Generated multiple lines of transgenic mice with confirmed mRNA expression of HLA-Ev (147) across organs, notably high in the heart and lungs.

Conclusions:

Codon optimization of HLA-Ev (147) for mammalian systems is crucial for developing effective transgenic animals for xenotransplantation.
The developed transgenic model provides a valuable tool for further xenotransplantation research.