Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantum estimation with state symmetry-induced optimal measurements.

Nature communications·2026

Same author

Low-Power and Flexible Optoelectronic Synapses Based on P3HT:Y6 Bulk Heterojunctions with Broadband Perception Capability.

The journal of physical chemistry letters·2026

Same author

Overexpression of a cytochrome P450 gene, CYP81Q32, endows resistance to HPPD-inhibiting herbicides in Leptochloa chinensis.

Pesticide biochemistry and physiology·2026

Same author

<i>Coptis chinensis</i> Franch. Suppresses Invasive Pulmonary Aspergillosis by Augmenting NADPH-Dependent Neutrophil Extracellular Traps via Dual Modulation of Complement Activation and Gut Microbiota.

Current issues in molecular biology·2026

Same author

Chimeric antigen receptor T-cell therapy in pediatric neurological autoimmune diseases: mechanisms, clinical applications, and future perspectives.

Frontiers in pediatrics·2026

Same author

Predicting Multidrug-Resistant Pneumonia: An Interpretable Machine Learning Model Validated in US and Chinese Patient Cohorts.

Infection and drug resistance·2026

Search research articles

Related Experiment Video

Updated: Mar 19, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Generating a host range-expanded recombinant baculovirus.

Chunfeng Wu^1,2, Zihao Deng¹, Zhao Long¹

¹State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.

Scientific Reports

|June 21, 2016

Summary

This summary is machine-generated.

Researchers engineered a baculovirus with an expanded host range by incorporating a specific gene from another virus. This modified virus, vAcRev, demonstrates enhanced infectivity in previously non-permissive insect hosts, paving the way for improved biopesticide applications.

More Related Videos

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Related Experiment Videos

Last Updated: Mar 19, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Area of Science:

Virology
Insect Pathology
Molecular Biology

Background:

Baculoviruses are valuable biopesticides but often have a narrow host range.
Understanding baculovirus host-range mechanisms is crucial for enhancing their pesticidal applications.
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Spodoptera exigua nucleopolyhedrovirus (SeMNPV) are important insect viruses.

Purpose of the Study:

To develop a baculovirus with an expanded host range.
To identify the genetic factors responsible for host-range expansion in baculoviruses.
To investigate the role of specific viral genes in overcoming host immune responses.

Main Methods:

Construction and plaque purification of a novel baculovirus recombinant, vAcRev, from AcMNPV and SeMNPV components.
Genome sequencing of the purified viral strain vAcRev.
RNA interference and ectopic expression assays to determine gene function.

Main Results:

vAcRev exhibited a broader host range than parental viruses, infecting a non-permissive host, Spodoptera litura.
Genome analysis revealed vAcRev is a mixture of two viruses, one with a modified genome containing SeMNPV genes.
A truncated Se-iap3 gene from SeMNPV was identified as the key factor responsible for host range expansion.

Conclusions:

The Se-iap3 gene plays a critical role in expanding the host range of baculoviruses.
Se-iap3 functions by inhibiting apoptosis and promoting viral replication in both permissive and non-permissive hosts.
This finding has significant implications for engineering baculoviruses with broader applications as biopesticides.