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Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis

R Q Yang1, H Teng1, X H Xu1

  • 1Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.

Genetics and Molecular Research : GMR
|June 21, 2016
PubMed

Insights

Aberrant microRNA (miRNA) expression in endometriosis influences angiogenesis. Specific miRNA level changes correlate with altered vascular endothelial growth factor A (VEGFA), epidermal growth factor receptor 2 (EGFR2), phosphatase and tensin homolog (PTEN), and C-X-C chemokine receptor type 4 (CXCR4) levels, impacting disease development.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Oncology

Background:

  • Endometriosis is characterized by aberrant angiogenesis.
  • MicroRNA (miRNA) dysregulation is implicated in various diseases, including endometriosis.
  • Understanding miRNA's role in endometriosis pathogenesis is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the aberrant microRNA (miRNA) expression profile in endometriosis.
  • To correlate miRNA expression with key angiogenic and fibrinolytic factors.
  • To elucidate the role of miRNA in the pathogenesis of endometriosis.

Main Methods:

  • MicroRNA expression was quantified using microRNA arrays and reverse-transcription quantitative polymerase chain reaction.
  • Levels of vascular endothelial growth factor A (VEGFA), epidermal growth factor receptor 2 (EGFR2), phosphatase and tensin homolog (PTEN), and C-X-C chemokine receptor type 4 (CXCR4) were measured using enzyme-linked immunosorbent assay.
  • Comparative analysis was performed between endometrial tissue and endometriotic lesions from the same patients.

Main Results:

  • Endometrial tissue exhibited significantly lower levels of miR-200b, miR-15a-5p, miR-19b-1-5p, miR-146a-5p, and miR-200c, and higher levels of miR-16-5p, miR-106b-5p, and miR-145-5p compared to endometriotic lesions.
  • Vascular endothelial growth factor A (VEGFA) was significantly upregulated in endometriotic tissues, while epidermal growth factor receptor 2 (EGFR2), phosphatase and tensin homolog (PTEN), and C-X-C chemokine receptor type 4 (CXCR4) were markedly downregulated.
  • These miRNA expression differences potentially modulate the expression of VEGFA, EGFR2, PTEN, and CXCR4.

Conclusions:

  • Aberrant miRNA expression profiles are characteristic of endometriosis.
  • MicroRNA dysregulation plays a significant role in the pathogenesis of endometriosis by affecting angiogenic and fibrinolytic factors.
  • Altered angiogenic activity in the eutopic endometrium may promote endometrial cell implantation at ectopic sites.

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