Atomic force microscopy combined with optical microscopy for cells investigation
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Updated: Mar 19, 2026

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology
Published on: July 5, 2022
Mariafrancesca Cascione1,2, Valeria de Matteis1, Rosaria Rinaldi1,2
1Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento Via Monteroni, 73100, Lecce, Italy.
Abstract:
This review reports on the combined use of the atomic force microscopy (AFM) and several type of optical/fluorescence/laser scanning microscopy for investigating cells. It is shown that the hybrid systems of AFM with optical-derived microscopies enable to study in detail cell surface properties (such as topography), their mechanical properties (e.g., Young's modulus) mechanotransduction phenomena and allow to gain insight into biological-related pathways and mechanisms in the complex nanoworld of cells. Microsc. Res. Tech. 80:109-123, 2017. © 2016 Wiley Periodicals, Inc.
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