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A replication-time-controlling sequence element in Schizosaccharomyces pombe.

Vishnu P Tripathi1, Dharani D Dubey2

  • 1Department of Biotechnology, Veer Bahadur Singh Purvanchal University, Jaunpur, UP, 222003, India.

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|June 22, 2016
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Summary
This summary is machine-generated.

A short fragment of the late-replication-enforcing element (LRE) in fission yeast enforces late replication timing for adjacent origins. This regulatory element functions in both native and ectopic chromosomal locations, impacting DNA replication timing.

Keywords:
2D gel analysisARS elementsFission yeastReplication originsReplication timing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic replication origins exhibit variable activity and replication timing.
  • Cis-acting regulatory sequences controlling chromosomal replication timing are not well understood.

Purpose of the Study:

  • To investigate the role of a specific DNA fragment in controlling replication timing.
  • To determine if a short fragment of the LRE can enforce late replication.

Main Methods:

  • Utilized the fission yeast Schizosaccharomyces pombe.
  • Tested a 133-bp fragment of the LRE (shLRE) in native and ectopic chromosomal locations.

Main Results:

  • The shLRE fragment was found to be necessary for enforcing late replication of adjoining origins.
  • This effect was observed in both native and early-replicating ectopic chromosomal sites.
  • The shLRE functions when positioned on either side of an origin.

Conclusions:

  • The shLRE is a functional cis-acting regulatory element.
  • It imposes late replication timing on chromosomal origins.
  • This finding contributes to understanding the regulation of DNA replication timing.