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The interaction between PmHtrA2 and PmIAP and its effect on the activity of Pm caspase

Anchulee Saleeart1, Karntichar Mongkorntanyatip1, Pakkakul Sangsuriya2

  • 1Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.

Fish & Shellfish Immunology
|June 22, 2016
PubMed

Insights

Black tiger shrimp (Penaeus monodon) apoptosis involves interactions between high temperature requirement A2 (PmHtrA2) and inhibitor of apoptosis protein (PmIAP). These proteins modulate Pm caspase activity, revealing a conserved cell death mechanism.

Area of Science:

  • Molecular Biology
  • Cell Death Research
  • Crustacean Biology

Background:

  • Apoptosis is crucial for multicellular organism development and homeostasis.
  • Key apoptotic proteins like PmHtrA2, PmIAP, and Pm caspase exist in Penaeus monodon.
  • The regulatory relationships among these shrimp apoptotic proteins remain unclear.

Purpose of the Study:

  • To investigate the interaction between PmHtrA2 and PmIAP in Penaeus monodon.
  • To elucidate the role of these interactions in regulating Pm caspase activity and apoptosis.
  • To determine if shrimp share conserved apoptosis regulation mechanisms with insects and mammals.

Main Methods:

  • Protein-protein interaction assays to confirm binding between PmHtrA2 and PmIAP.
  • Domain mapping to identify the specific region (BIR2 domain of PmIAP) involved in binding.
  • In vitro assays to assess the effect of PmHtrA2 and PmIAP on Pm caspase activity, including the role of the IBM peptide.

Main Results:

  • PmHtrA2 directly interacts with PmIAP, mediated by the BIR2 domain of PmIAP.
  • The BIR2 domain of PmIAP inhibits Pm caspase activity.
  • PmHtrA2's IBM peptide disrupts PmIAP's inhibition of Pm caspase, suggesting PmHtrA2 activates apoptosis.

Conclusions:

  • Penaeus monodon exhibits a conserved apoptosis regulatory pathway involving HtrA2 and IAP proteins.
  • This pathway modulates caspase-3 activity, similar to mechanisms observed in insects and mammals.
  • The findings provide insights into the molecular mechanisms controlling cell death in shrimp.
Keywords:
ApoptosisPenaeus monodonPm caspasePmHtrA2PmIAP

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