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Updated: Mar 19, 2026

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
Published on: February 7, 2019
On-line comprehensive two-dimensional normal-phase liquid chromatography×reversed-phase liquid chromatography for
Jia-Fu Li1, Hua Fang2, Xia Yan1
1School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
Abstract:
An on-line comprehensive preparative two-dimensional normal-phase liquid chromatography×reversed-phase liquid chromatography (2D NPLC×RPLC) system was constructed with a newly developed vacuum evaporation assisted adsorption (VEAA) interface, allowing fast removal of NPLC solvent in the vacuum condition and successfully solving the solvent incompatibility problem between NPLC and RPLC. The system achieved on-line solvent exchange within the two dimensions and its performance was illustrated by gram-scale isolation of crude extract from the venom of Bufo bufo gargarizans. Within separation time of ∼20h, 19 compounds were obtained with high purity in a single run. With the VEAA interface, the 2D system exhibited apparent advantages in separation efficiency and automation compared with conventional methods, indicating its promising application in the routine separation process for complicated natural products.
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