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Updated: Mar 19, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Baeyer-Villiger oxidations: biotechnological approach
Marek Bučko1, Peter Gemeiner2, Andrea Schenkmayerová1
1Department of Glycobiotechnology, Institute of Chemistry - Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia.
Abstract:
Baeyer-Villiger monooxygenases (BVMOs) are a very well-known and intensively studied class of flavin-dependent enzymes. Their substrate promiscuity, high chemo-, regio-, and enantioselectivity are prerequisites for the use in synthetic chemistry and should pave the way for successful industrial processes. Nonetheless, only a very limited number of industrial relevant transformations are known, mainly due to the lack of BVMOs stability and cofactor dependency. In this review, we focus on novel BVMO-mediated transformations, BVMOs in cascade type reactions, potential industrial applications, and how limitations have been tackled by the community. Special attention will be put on whole-cell immobilization strategies. We emphasize to bridge recent developments in fundamental research to industrial applications.
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