Related Experiment Video
Updated: Mar 19, 2026
08:32
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
Published on: May 23, 2025
1.4K
The applications of CRISPR screen in functional genomics
Briefings in Functional Genomics
|June 23, 2016
Summary
CRISPR/Cas9 gene editing technology offers an easier, efficient, and less cytotoxic method for biological research. This review highlights CRISPR screens in cancer research, covering gene editing, model creation, and therapy.
Area of Science:
- Genomic science
- Molecular biology
- Biotechnology
Background:
- CRISPR/Cas9 is a programmable genome editing technology derived from bacterial immune systems.
- It offers advantages over traditional methods, being easier, more efficient, and less cytotoxic.
- The technology has revolutionized biological research, making precise genome editing routine.
Purpose of the Study:
- To review the applications of CRISPR screening in cancer research.
- To discuss practical aspects of designing CRISPR screens.
- To outline future directions in genome editing technology.
Main Methods:
- Focus on CRISPR screening methodologies.
- Review of CRISPR/Cas9 applications in cancer research areas.
- Discussion of screen design principles.
Main Results:
- CRISPR/Cas9 is widely applied in cancer research, including tumor gene studies and model development.
- CRISPR screens are valuable for identifying genes related to tumor phenotype and drug resistance.
- The technology facilitates advancements in cancer gene therapy.
Conclusions:
- CRISPR screening is a powerful tool in the rapidly evolving field of cancer research.
- Further development in screen design and application is anticipated.
- CRISPR technology continues to expand the possibilities in genome editing.
Related Concept Videos
02:46Genetic Screens
5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K
01:59CRISPR
58.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.8K
01:59CRISPR
18.8K
18.8K
01:28CRISPR/Cas9 Genome Editing
2.5K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.5K
02:53CRISPR and crRNAs
19.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.5K