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Determination of Sterigmatocystin in Infant Cereals from Hangzhou, China
Shaoying Liu1, Jicai Fan1, Xihui Huang1
1Hangzhou Center for Disease Control and Prevention, Laboratory of Chemistry and Physics, Mingshi Rd No. 568, Hangzhou 310021, People's Republic of China.
Abstract:
A simple method was developed for the determination of sterigmatocystin in infant cereals. The method consists of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction approach and cleanup with an SPE HLB cartridge. After purification for separation and quantification of sterigmatocystin, HPLC with UV detection and diode-array detection (DAD) was used at sterigmatocystin levels >10 μg/kg, whereas ultra-performance LC with electrospray ionization (ESI) and tandem MS (MS/MS) was used at levels <10 μg/kg. The compound was determined by UV-DAD at 325 nm and by ESI-MS/MS in the positive ionization mode. Good r(2) values (≥0.99) were found with both UV-DAD and ESI-MS/MS, and satisfactory recoveries (84.8-96.2%) of sterigmatocystin in infant cereals were demonstrated, with RSD values ≤11.43%. The developed analytical method was used for the determination of sterigmatocystin in infant cereals from Hangzhou, China.
