Related Experiment Video
Updated: Mar 19, 2026
Laser Doppler Perfusion Imaging in the Mouse Hindlimb
Published on: April 18, 2021
Microcirculation in healing and healthy Achilles tendon assessed with invasive laser doppler flowmetry
Erica Domeij Arverud1, Olof Persson-Lindell2, Fredrik Sundquist2
1Department of Orthopedic Surgery, Danderyd Hospital, Stockholm, Sweden.
Introduction:
Achilles tendon (AT) rupture exhibits a prolonged healing process with varying clinical outcome. Reduced blood flow to the AT has been considered an underlying factor to AT rupture (ATR) and impaired healing. In vivo measurements using laser Doppler flowmetry (LDF) may be a viable method to assess blood flow in healthy and healing AT.
Methods:
29 persons were included in the study; 9 being ATR patients and 20 healthy subjects without any prior symptoms from the AT. Invasive LDF was used to determine the post-occlusive reactive hyperemia (PORH) in the paratenon after 15 minutes of occlusion of the lower extremities. ATR patients were examined two weeks post-operatively.
Results:
LDF-assessments demonstrated a significantly different (p < 0.001) PORH response in the healing- versus intact- and control AT. In the healing AT, a slow, flattened PORH was observed compared to a fast, high peak PORH in intact, healthy AT.
Conclusion:
in vivo LDF appears to be a feasible method to assess alterations in blood flow in healing and intact AT. The healing ATs capability to react to an ischemic period is clearly impaired, which may be due to the trauma at injury and/or surgery or degenerative changes in the tendon.
