Data on physicochemical properties of LPEI 25 kDa, PEI"Max" 40 kDa and PEIpro™
Laurence Delafosse1, Ping Xu2, Yves Durocher1
1Département de biochimie et médecine moléculaire, Faculté de Médecine, Université de Montréal, QC, Canada; National Research Council of Canada, Building Montreal-Royalmount, 6100 Royalmount Avenue, Montreal, QC, Canada H4P 2R2.
Abstract:
The data presented in this article are related to the research article entitled "Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells" (Delafosse et al., 2016 [1]). Polyethylenimine is a cationic polymer whose linear form has been described as the most efficient to transfect a wide range of cell lines and thus is broadly used in transient gene expression. Data presented in this article compares apparent size and polydispersity as determined by size exclusion chromatography of three commercially available linear PEIs, namely LPEI, PEI"Max" and PEIpro™. Impact of those features on plasmid DNA affinity was established by plasmid DNA agarose gel migration assay.
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