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Quantitative assessment of epidermal Langerhans cells using automated image analysis
J Smolle1, R Hofmann-Wellenhof1
1Department of Dermatology, University of Graz, Austria.
Background/Aims:
Langerhans cells play a central role In the skin immune system. Quantitative changes have been observed in a variety of dermatological conditions. This paper presents a user-independent automated measurement procedure for Langerhans cells in vertical skin sections.
Methods:
Frozen sections were stained immunohistochemically with a CD1 monoclonal antibody. Counterstaining was performed with Mayer's hematoxylin. An image analysis procedure was developed, which automatically recognizes the area occupied by the epidermis and the CD1 -positive structures, respectively.
Results:
The procedure was tested on specimens of normal skin and of chronic cutaneous lupus erythematosus. The results significantly reflect the decrease of Langerhans cells in the latter condition.
Conclusion:
The proposed image analysis program facilitates a fully automated measurement of immunohistochemically stained epidermal structures in vertical skin sections.
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