Large-Scale Mesenchymal Stem/Stromal Cell Expansion: A Visualization Tool for Bioprocess Comparison.

Area of Science:

• Biotechnology
• Regenerative Medicine
• Cell Therapy Manufacturing

Background:

• Cell-based therapies require scalable and cost-effective cell expansion.
• Existing literature lacks standardized methods for comparing diverse cell expansion processes.
• This hinders efficient process selection and optimization for clinical translation.

Purpose of the Study:

• To systematically review mesenchymal stromal cell (MSC) expansion literature.
• To develop an interactive visualization tool for comparing MSC expansion processes.
• To define bioprocess metrics for ensuring comparability between different methods.

Main Methods:

• Systematic literature review of MSC expansion processes.
• Development of a computational visualization tool.
• Creation of standardized bioprocess metrics.
• Data aggregation of 73 expansion processes across various MSC types and culture vessels.

Main Results:

• A comprehensive data repository of 73 human MSC expansion processes was established.
• An interactive visualization tool was created for process comparison and benchmarking.
• Defined bioprocess metrics enable standardized evaluation of cell expansion efficiency.
• The tool serves as a reference for assessing cell expansion process performance.

Conclusions:

• Standardized metrics and visualization tools are crucial for comparing cell expansion processes.
• This approach facilitates a better understanding of parameters influencing cell culture.
• Enables rational selection and design of bioprocesses for cell therapy manufacturing.
• Aids in advancing the clinical and commercial translation of cell-based therapies.