Related Experiment Video
Updated: Mar 19, 2026
08:21
Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
6.0K
Massiliapinisoli sp. nov., isolated from forest soil
Khulan Altankhuu1, Jaisoo Kim1
1Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Republic of Korea.
Summary
A novel bacterial species, Massilia pinisoli T33T, was identified from South Korean forest soil. This Gram-negative bacterium exhibits unique physiological and biochemical characteristics, distinguishing it as a new member of the Massilia genus.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Environmental Microbiology
Background:
- The genus Massilia comprises aerobic, Gram-negative bacteria found in various environments.
- Accurate taxonomic classification is crucial for understanding microbial diversity and ecological roles.
Purpose of the Study:
- To characterize and classify a novel bacterial isolate, designated T33T, obtained from forest soil.
- To determine the phylogenetic position and taxonomic distinctiveness of strain T33T within the genus Massilia.
Main Methods:
- Isolation and cultivation of bacteria from forest soil.
- Gram staining, motility tests, and biochemical characterization (catalase, oxidase).
- 16S rRNA gene sequencing, phylogenetic analysis, DNA-DNA hybridization, and determination of DNA G+C content.
Main Results:
- Strain T33T is a Gram-negative, aerobic, motile, rod-shaped bacterium.
- Phylogenetic analysis based on 16S rRNA gene sequences showed T33T belongs to the genus Massilia, with highest similarity (98.7%) to Massilia niastensis KACC 12599T.
- DNA-DNA hybridization revealed less than 70% relatedness to closely related type strains, and the DNA G+C content was 69.4 mol%.
- Major polar lipids included phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, with ubiquinone Q-8 as the predominant respiratory quinone.
- Major fatty acids were summed feature 3, summed feature 8, and C16:0.
Conclusions:
- Strain T33T represents a novel species within the genus Massilia.
- Based on its distinct phylogenetic and chemotaxonomic properties, Massilia pinisoli sp. nov. is proposed.
- The type strain is T33T (=KACC 18748T=KEMB 9005-368T=JCM 31316T).
Related Concept Videos
01:25Microbial Mats
6
Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
6
01:29Soil Microbial Ecology
1
Soil microbial ecology is defined by highly diverse, spatially structured communities that drive nutrient cycling, organic matter turnover, and overall ecosystem stability. Although a gram of soil can contain thousands of bacterial and archaeal taxa, the ecological processes they mediate are even more crucial for sustaining terrestrial life.Microhabitats and NichesSoil is a heterogeneous mixture of minerals, organic matter, water, and air. Microbes inhabit distinct microhabitats formed by...
1
01:24Bacterial Phylum Tenericutes
574
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
574
01:28Fungal Phylum Microsporidia
679
Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...
679
01:30Marine Microbial Ecology
1
Marine microbial ecosystems are shaped by distinct physicochemical limits, including high salinity, low nutrient availability, and fluctuating oxygen levels. These conditions favor smaller microbial cell sizes, which maximize their surface-to-volume ratio for efficient nutrient uptake.Microbial activity and community composition are closely linked to biogeochemical cycles, particularly in dynamic environments like estuaries, where halotolerant microbes thrive in response to variable salinity...
1
01:30Bacterial Phylum Actinobacteria
809
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
809