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Published on: September 15, 2018
Microarray Expression Profile of Circular RNAs in Heart Tissue of Mice with Myocardial Infarction-Induced Heart
Hong-Jin Wu1, Cheng-Ying Zhang, Sai Zhang
1Beijing Haidian Hospital, Haidian Section of Peking University Third Hospital, Beijing, China.
Insights
Circular RNAs (circRNAs) show altered expression in the heart during heart failure (HF) following myocardial infarction (MI). These findings suggest circRNAs could be key biomarkers for HF diagnosis and treatment.
Area of Science:
- Cardiovascular Biology
- Molecular Biology
- Genomics
Background:
- Myocardial infarction (MI) is a major cause of heart failure (HF) and mortality.
- Atherosclerosis underlies MI, leading to progressive cardiac dysfunction.
- Understanding molecular changes in HF is crucial for developing new therapies.
Purpose of the Study:
- To investigate global changes in circular RNA (circRNA) expression in heart tissue during MI-induced HF.
- To characterize the role of circRNAs in the pathophysiology of heart failure.
- To identify potential circRNA biomarkers for HF.
Main Methods:
- Utilized a mouse model of post-myocardial infarction (MI) induced heart failure (HF).
- Employed microarray assays to profile circRNA transcriptomes in cardiac tissue.
- Validated differential circRNA expression using quantitative PCR.
Main Results:
- Identified numerous deregulated circular RNAs (circRNAs) in the heart during HF.
- Confirmed significant changes in specific circRNA expression levels.
- CircRNA deregulation suggests a potential role in HF development and progression.
Conclusions:
- Distinct expression patterns of circRNAs were observed during HF.
- circRNAs may actively respond to cardiac stress.
- circRNAs show promise as diagnostic and therapeutic biomarkers for heart failure.
Background/Aims:
Myocardial infarction (MI) is a serious complication of atherosclerosis associated with increasing mortality attributable to heart failure. This study is aimed to assess the global changes in and characteristics of the transcriptome of circular RNAs (circRNAs) in heart tissue during MI induced heart failure (HF).
Methods:
Using a post-myocardial infarction (MI) model of HF in mice, we applied microarray assay to examine the transcriptome of circRNAs deregulated in the heart during HF. We confirmed the changes in circRNAs by quantitative PCR.
Results:
We revealed and confirmed a number of circRNAs that were deregulated during HF, which suggests a potential role of circRNAs in HF.
Conclusions:
The distinct expression patterns of circulatory circRNAs during HF indicate that circRNAs may actively respond to stress and thus serve as biomarkers of HF diagnosis and treatment.
