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Related Experiment Video

Updated: Mar 19, 2026

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Wrinkled-Surface Mediated Reverse Transfection Platform for Highly Efficient, Addressable Gene Delivery.

Hyunwoo Joo1, Jisoo Shin2, Seung-Woo Cho2

  • 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-338, Republic of Korea.

Advanced Healthcare Materials
|June 24, 2016
PubMed
Summary

A new wrinkled-surface platform enhances gene delivery efficiency for long-term applications. This novel reverse transfection method maximizes cell-substrate contact for improved delivery rates and multiplexed material introduction.

Keywords:
reverse transfectionself-forming siRNA nanoparticlewrinkled surface

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Area of Science:

  • Biotechnology and Bioengineering
  • Cell Biology
  • Materials Science

Background:

  • Gene delivery is crucial for research and therapeutics.
  • Current methods face challenges in efficiency and long-term expression.
  • Optimizing cell-substrate interaction is key for transfection success.

Purpose of the Study:

  • To develop a novel reverse transfection platform for enhanced gene delivery.
  • To investigate the impact of surface topography on transfection efficiency.
  • To demonstrate multiplexed and addressable delivery of exogenous materials.

Main Methods:

  • Development of a wrinkled-surface substrate for cell culture.
  • Utilizing reverse transfection principles to mediate gene delivery.
  • Incorporation of microwell structures for spatial control.
  • Quantification of gene delivery efficiency and long-term expression.

Main Results:

  • The wrinkled-surface platform significantly increased gene delivery rates.
  • Enhanced cell-substrate contact area correlated with higher transfection efficiency.
  • Multiplexed and addressable delivery of exogenous materials was successfully achieved using microwell structures.
  • The platform demonstrated potential for highly efficient long-term gene delivery.

Conclusions:

  • The wrinkled-surface reverse transfection platform offers a promising approach for efficient and long-term gene delivery.
  • Surface topography plays a critical role in optimizing cell-substrate interactions for transfection.
  • The developed platform enables precise control over material delivery for various applications.