Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

4.4K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
4.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

1.8K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
1.8K
Deactivation Processes: Jablonski Diagram01:25

Deactivation Processes: Jablonski Diagram

2.1K
Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The energy-level diagram, or Jablonski diagram, is a graphical representation of these interactions, illustrating the various states and transitions a molecule can undergo. In a typical Jablonski diagram, the lowest horizontal line represents the ground-state energy of the molecule, which is usually a singlet state. This state represents the energies...
2.1K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.2K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.2K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.8K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Research on real-time detection and staging technology for pressure injuries in critically ill patients based on the YOLOv8 deep learning model.

Frontiers in public health·2026
Same author

An ensemble pipeline, PhageHost, for phage tail fiber discovery and accurate Klebsiella pneumoniae host prediction using protein language models.

Cell reports·2026
Same author

Enhancing charge separation efficiency in photocatalytic hydrogen evolution <i>via</i> a synergistic strategy based on point/interface dual-defect engineering in Schottky heterojunctions.

Nanoscale·2026
Same author

Shear wave elastography combined with serum IL-6 and MMP-9 for evaluating disease activity in Takayasu arteritis with carotid artery involvement.

Clinical rheumatology·2026
Same author

Interpretation of Comparative Outcomes in Bone-Metastatic Clear Cell Renal Cell Carcinoma.

Clinical genitourinary cancer·2026
Same author

First-phase ejection fraction for evaluating early left ventricular systolic function in patients with cirrhosis.

The Journal of international medical research·2026
Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026
Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026
Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026
Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026
Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026
Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Mar 19, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.3K

Gelation process visualized by aggregation-induced emission fluorogens.

Zhengke Wang1,2, Jingyi Nie1,2, Wei Qin3

  • 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.

Nature Communications
|June 24, 2016
PubMed
Summary
This summary is machine-generated.

This study reveals the chitosan gelation process in a lithium hydroxide-urea aqueous solvent system using advanced imaging. We elucidate the formation of junction points, including hydrogen bonds and crystalline structures, crucial for hydrogel properties.

More Related Videos

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.9K
Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.7K

Related Experiment Videos

Last Updated: Mar 19, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.3K
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.9K
Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.7K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Biochemistry

Background:

  • Alkaline-urea aqueous solvent systems offer a novel route for polysaccharide utilization.
  • Chitosan hydrogels exhibit unique properties, but their gelation process is not fully understood.
  • Existing studies lack direct observation of the entire gelation process.

Purpose of the Study:

  • To investigate the complete gelation process of chitosan in a LiOH-urea aqueous system.
  • To elucidate the mechanism of junction point formation during gelation.
  • To understand the role of hydrogen bonds and crystalline structures in chitosan hydrogels.

Main Methods:

  • Aggregation-induced emission (AIE) fluorescent imaging for real-time observation.
  • Pseudo in situ investigations to complement imaging data.
  • Analysis of hydrogen bonding and crystalline structures.

Main Results:

  • Direct visualization of the entire chitosan gelation process was achieved.
  • The mechanism of junction point formation, involving hydrogen bonds and crystalline structures, was proposed.
  • Unique properties of the resultant chitosan hydrogel were linked to the gelation mechanism.

Conclusions:

  • The study provides fundamental insights into chitosan gelation in LiOH-urea aqueous systems.
  • AIE imaging is a powerful tool for studying dynamic gelation processes.
  • Understanding gelation mechanisms is key to optimizing chitosan-based hydrogel materials.