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Updated: Mar 19, 2026
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2
Qiao Li1,2, Pan Chen3, Zhaoyang Zeng4,5,6
1The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Abstract:
Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2), a member of the TSC22D family, has been implicated as a tumor-associated gene, but its function remains unknown. To further explore its biological role, yeast two-hybrid screening combined with multiple bioinformatics tools was used to identify 44 potential interacting partners of the TSC22D2 protein that were mainly involved in gene transcription, cellular metabolism, and cell cycle regulation. The protein WD repeat domain 77 (WDR77) was selected for further validation due to its function in the cell cycle and tumor development, as well as its high detection frequency in the yeast two-hybrid assay. Immunoprecipitation and immunofluorescence experiments confirmed an interaction between the TSC22D2 and WDR77 proteins. Our work greatly expands the putative protein interaction network of TSC22D2 and provides deeper insight into the biological functions of the TSC22D2 and WDR77 proteins.
Insights
Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2) interacts with WD repeat domain 77 (WDR77). This study identifies new protein partners, offering insights into TSC22D2 and WDR77 functions in cell cycle regulation and tumor development.
Area of Science:
- Molecular Biology
- Cancer Research
- Protein Interactions
Background:
- Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2) is a tumor-associated gene with an unknown function.
- Understanding TSC22D2's biological role is crucial for cancer research.
Purpose of the Study:
- To identify and characterize the protein interaction partners of TSC22D2.
- To elucidate the biological functions of TSC22D2 and its interacting proteins, particularly in relation to the cell cycle and tumor development.
Main Methods:
- Yeast two-hybrid screening was employed to identify potential TSC22D2 interacting proteins.
- Bioinformatics tools were utilized to analyze the identified partners.
- Immunoprecipitation and immunofluorescence assays were performed for validation of protein interactions.
Main Results:
- Forty-four potential interacting partners of TSC22D2 were identified, primarily involved in gene transcription, cellular metabolism, and cell cycle regulation.
- WD repeat domain 77 (WDR77) was validated as a direct interacting partner of TSC22D2.
- The interaction between TSC22D2 and WDR77 was confirmed through co-immunoprecipitation and immunofluorescence.
Conclusions:
- This study expands the known protein interaction network of TSC22D2.
- The findings provide novel insights into the molecular mechanisms underlying the functions of TSC22D2 and WDR77 in cellular processes, including cell cycle regulation and potentially tumor development.
