Related Experiment Video

Updated: Mar 19, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

MicroRNA-202 induces cell cycle arrest and apoptosis in lung cancer cells through targeting cyclin D1

J Jiang1, J Huang, X-R Wang

  • 1Department of Respiratory Medicine, Wuhan Central Hospital, Wuhuan, China. wangxinrong223@163.com.

European Review for Medical and Pharmacological Sciences
|June 25, 2016
PubMed
Abstract

Insights

MicroRNA-202 (miR-202) is downregulated in lung cancer. Restoring miR-202 inhibits cancer cell proliferation by targeting cyclin D1, suggesting its potential as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development.
  • Dysregulation of specific miRNAs is implicated in tumorigenesis.
  • The role of miR-202 in human lung cancer proliferation requires further investigation.

Purpose of the Study:

  • To investigate the function of miR-202 in the proliferation of human lung cancer cells.
  • To determine the expression levels of miR-202 in lung cancer tissues.
  • To elucidate the molecular mechanism underlying miR-202's function in lung cancer.

Main Methods:

  • Real-time PCR for miR-202 expression analysis.
  • CCK-8 assay and flow cytometry for cell proliferation and cell cycle analysis.
  • Western blot and luciferase activity assays to determine target interactions and protein expression.

Main Results:

  • MiR-202 expression was significantly reduced in lung cancer tissues compared to adjacent normal tissues.
  • Overexpression of miR-202 suppressed lung cancer cell proliferation, induced G0/G1 cell cycle arrest, and promoted apoptosis.
  • MiR-202 directly targets the 3' untranslated region (UTR) of cyclin D1 mRNA, inhibiting its protein expression.

Conclusions:

  • MiR-202 acts as a tumor suppressor gene in lung cancer by targeting cyclin D1.
  • Downregulation of miR-202 is a common event in lung cancer.
  • Modulating miR-202 levels holds potential as a novel therapeutic strategy for lung cancer.

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

487

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.1K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K