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Breath Collection from Children for Disease Biomarker Discovery
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Development of a Sampling Collection Device with Diagnostic Procedures.

Jhih-Yan Cheng, Mow-Jung Feng1, Chia-Chi Wu1

  • 1Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch , Taoyuan 333, Taiwan.

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|June 25, 2016
PubMed
Summary
This summary is machine-generated.

A new dual-material device effectively collects cervicovaginal fluid for disease diagnosis. This noninvasive method improves pathogen detection and female genital cancer screening, enhancing diagnostic accuracy.

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Area of Science:

  • Gynecology
  • Biomedical Engineering
  • Clinical Diagnostics

Background:

  • Cervicovaginal fluid is crucial for diagnosing female genital diseases.
  • Current collection devices are inadequate, hindering accurate testing.
  • Improved sampling methods are needed for rapid and efficient disease detection.

Purpose of the Study:

  • To develop a user-friendly sampling device for cervicovaginal fluid.
  • To eliminate macromolecular interference in collected specimens.
  • To enable accurate diagnosis of female genital diseases.

Main Methods:

  • A dual-material (nonwoven fabric and cotton) collection device was developed.
  • The device was tested by 103 patients for cervicovaginal fluid collection.
  • Collected specimens were analyzed using paper-based assays, ELISA, and qNano.

Main Results:

  • The developed device successfully collected cervicovaginal fluid from 103 patients.
  • Specimens were used for pathogen infection markers (glycogen, lactate, pH).
  • Female genital cancer was screened by analyzing interleukin 6 and microvesicle concentrations.

Conclusions:

  • A noninvasive, dual-material device offers an effective method for cervicovaginal fluid collection.
  • This approach facilitates accurate diagnosis of female genital diseases, including cancer.
  • The device shows potential for improving screening and diagnostic capabilities in women's health.