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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

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Molecular Characteristics of Methicillin-Resistant Staphylococcus epidermidis on the Abdominal Skin of Females before

Pin-Jia Wang1,2, Cheng-Bin Xie3, Feng-Hui Sun4

  • 1Medical Genetics, West China Shool of Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. pinjiawong@foxmail.com.

International Journal of Molecular Sciences
|June 25, 2016
PubMed

Insights

Methicillin-resistant Staphylococcus epidermidis (MRSE) is common on abdominal skin before surgery. These resistant strains show diverse genetic profiles and unfavorable antimicrobial susceptibility patterns, posing a risk for surgical site infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Staphylococcus epidermidis, particularly methicillin-resistant strains (MRSE), can cause surgical site infections.
  • MRSE may act as a reservoir for staphylococcal cassette chromosome mec (SCCmec) in S. aureus.
  • Understanding MRSE prevalence and characteristics on patient skin is crucial for infection control.

Purpose of the Study:

  • To determine the prevalence of MRSE on the abdominal skin of females undergoing laparotomy.
  • To characterize the molecular features of MRSE isolates.
  • To assess the antimicrobial susceptibility patterns of MRSE.

Main Methods:

  • Detection of MRSE using mecA gene.
  • Antimicrobial susceptibility testing via broth microdilution.
  • Molecular typing including SCCmec and pulsed field gel electrophoresis (PFGE).

Main Results:

  • MRSE was detected in 54 out of 157 isolates (34.4%).
  • All MRSE isolates exhibited unfavorable antimicrobial susceptibility; 42.6% were multi-drug resistant.
  • High diversity in SCCmec types (predominantly type IVa) and PFGE patterns was observed, with no identical pulsotypes.

Conclusions:

  • Methicillin-resistant Staphylococcus epidermidis is frequently found on the abdominal skin of females prior to laparotomy.
  • MRSE isolates display significant antimicrobial resistance and genetic diversity.
  • Resistance profiles did not appear to correlate with SCCmec or PFGE types for common antibiotics.
Keywords:
PFGESCCmecantibiotic resistantlaparotomymethicillin-resistant Staphylococcus epidermidisskin

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