Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Direct-Acting Cholinergic Agonists: Pharmacokinetics

Direct-Acting Cholinergic Agonists: Pharmacokinetics

Direct-acting cholinergic agonists, such as synthetic choline esters and naturally occurring alkaloids, exert their effects by enhancing the actions of acetylcholine and stimulating the parasympathetic nervous system. Synthetic choline esters share structural similarities with acetylcholine. For example, they have a positively charged quaternary ammonium or onium group, contributing to their hydrophilic characteristics. As a result, they are poorly absorbed in the body through oral...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of pulsed electric field technology on whey protein isolate.

Journal of dairy science·2026

Same author

Influence of Pulsed Electric Field Technology on Functionality and Protein Structure of Evaporated Skim Milk and Nonfat Dry Milk.

International journal of molecular sciences·2026

Same author

Effects of Pulsed Electric Field Technology on Whey Protein Concentrate.

Molecules (Basel, Switzerland)·2026

Same author

An mRNA vaccine encoding the Ebola virus glycoprotein induces high neutralizing antibody titers and provides strong protection against lethal infections in mouse models.

Frontiers in immunology·2026

Same author

Evaluation of Methods to Quantify Sialic Acid on Glycomacropeptide.

Foods (Basel, Switzerland)·2025

Same author

Automated Spectral Preprocessing via Bayesian Optimization for Chemometric Analysis of Milk Constituents.

Foods (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Mar 19, 2026

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Published on: December 23, 2022

Cyclopamine bioactivity by extraction method from Veratrum californicum.

Matthew W Turner¹, Roberto Cruz², Jared Mattos²

¹Department of Chemistry and Biochemistry, Boise State University, 1910 University Drive, Boise, ID 83725, United States; Biomolecular Sciences Ph.D. Program, Boise State University, 1910 University Drive, Boise, ID 83725, United States.

Bioorganic & Medicinal Chemistry

|June 25, 2016

Summary

Ethanol soak is the best method for extracting biologically active cyclopamine from Veratrum californicum. This steroidal alkaloid inhibits the Hedgehog (Hh) signaling pathway, which is important in development and cancer.

Keywords:

Cyclopamine Hedgehog signaling Steroidal alkaloids Veratrum californicum

More Related Videos

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

Related Experiment Videos

Last Updated: Mar 19, 2026

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Published on: December 23, 2022

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

Area of Science:

Pharmacognosy
Molecular Biology
Biochemistry

Background:

Veratrum californicum produces cyclopamine, a steroidal alkaloid.
Cyclopamine inhibits the Hedgehog (Hh) signaling pathway.
Hh pathway dysregulation is implicated in cancer progression.

Purpose of the Study:

To evaluate eight extraction methods for cyclopamine from Veratrum californicum.
To correlate extraction efficiency with biological activity.
To identify the optimal method for obtaining active cyclopamine.

Main Methods:

Extraction of cyclopamine from V. californicum roots and rhizomes using eight different methods.
Assay of biological activity using Shh-Light II cells and a Dual-Glo® Luciferase Assay System.
Quantification of alkaloid recovery.

Main Results:

Alkaloid recovery varied from 0.39 to 8.03 mg/g.
Ethanol soak yielded the highest recovery of biologically active cyclopamine.
Acidic ethanol and supercritical extractions resulted in degraded or contaminated cyclopamine with reduced Hh inhibitory activity.

Conclusions:

Ethanol soak is the most effective method for extracting biologically active cyclopamine.
Extraction method significantly impacts the purity and Hh signaling inhibitory activity of cyclopamine.
Optimized cyclopamine extraction is crucial for its therapeutic potential in targeting Hh-driven cancers.