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Updated: Mar 19, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Microglia development follows a stepwise program to regulate brain homeostasis.

Orit Matcovitch-Natan¹, Deborah R Winter², Amir Giladi²

¹Department of Immunology, Weizmann Institute of Science, Rehovot, Israel. Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.

Science (New York, N.Y.)

|June 25, 2016

Summary

This summary is machine-generated.

Microglia, the brain's immune cells, develop in three distinct stages synchronized with brain maturation. Disruptions to this process may link to neurodevelopmental disorders.

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Last Updated: Mar 19, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

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Published on: January 30, 2014

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Culturing Microglia from the Neonatal and Adult Central Nervous System

Culturing Microglia from the Neonatal and Adult Central Nervous System

Published on: August 9, 2013

Area of Science:

Neuroscience
Immunology
Developmental Biology

Background:

Microglia are crucial myeloid cells in the central nervous system, vital for brain maintenance and disease.
The precise regulatory mechanisms governing microglia development throughout the brain's lifespan remain incompletely understood.

Purpose of the Study:

To elucidate the regulatory dynamics and developmental stages of microglia within the central nervous system.
To identify the genetic and environmental factors influencing microglia development and their potential link to neurodevelopmental disorders.

Main Methods:

Genome-wide chromatin and expression profiling.
Single-cell transcriptomic analysis across developmental stages.
Gene knockout studies (Mafb) and environmental perturbation models (microbiome, prenatal immune activation).

Main Results:

Identification of three distinct microglia developmental stages: early, pre-, and adult microglia, each with unique regulatory circuits.
Demonstration that knockout of the MAFB transcription factor disrupts adult microglia development.
Environmental factors like microbiome changes and prenatal immune activation impact developmental genes and immune pathways in microglia.

Conclusions:

Microglia exhibit a stepwise developmental program tightly regulated and synchronized with brain development.
This developmental program integrates immune response pathways, suggesting a potential role in neurodevelopmental disorders.