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Published on: July 28, 2016
Serum myeloperoxidase (MPO) activity, oxidative and antioxidative parameters in operating room personnel
Muhammed Bilal Cegin1, Mehmet Aslan2, Ugur Goktas1
1Department of Anaesthesiology and Reanimation, Turkey.
Objective:
To investigate the effects of occupational exposure to anaesthetic gases on myeloperoxidase activity, oxidative and antioxidative parameters in operating room personnel.
Methods:
The cross-sectional study was conducted at Yuzuncu Yil University, Van, Turkey, in May 2011, and comprised equal number of operating room and non-operating room personnel. Serum myeloperoxidase activity, sulfhydryl group levels, lipid hydroperoxide levels and catalase activity were determined. SPSS 11 was used for data analysis.
Results:
There were 64 subjects; 32(50%) each in the two groups. Myeloperoxidase activity and lipid hydroperoxide levels were significantly higher in operating room personnel than in the non-operating room personnel (p<0.001; p<0.001), while catalase activity and sulfhydryl group levels were significantly lower (p<0.009; p<0.003). Catalase activity negatively correlated with lipid hydroperoxide levels in operating room personnel (r=-0.293; p=0.018). Myeloperoxidase activity negatively correlated with sulfhydryl group levels in operating room personnel (r=-0.267; p=0.031).
Conclusions:
Operating room personnel exhibited higher oxidative stress, which may be due to the oxidative effect of anaesthetic gases.
Insights
Operating room staff exposed to anesthetic gases show increased oxidative stress. This occupational exposure elevates myeloperoxidase and lipid hydroperoxide levels while decreasing catalase and sulfhydryl groups.
Area of Science:
- Occupational Health
- Biochemistry
- Environmental Medicine
Background:
- Operating room personnel face potential exposure to anesthetic gases.
- Anesthetic gases may induce oxidative stress.
- Assessing oxidative and antioxidative markers is crucial for understanding health impacts.
Purpose of the Study:
- To investigate the effects of occupational exposure to anesthetic gases on myeloperoxidase activity.
- To evaluate oxidative and antioxidative parameters in operating room personnel.
Main Methods:
- A cross-sectional study involving 64 operating room and non-operating room personnel.
- Measurement of serum myeloperoxidase activity, sulfhydryl group levels, lipid hydroperoxide levels, and catalase activity.
- Statistical analysis using SPSS 11.
Main Results:
- Operating room personnel had significantly higher myeloperoxidase activity and lipid hydroperoxide levels (p<0.001 for both).
- Operating room personnel showed significantly lower catalase activity and sulfhydryl group levels (p<0.009 and p<0.003, respectively).
- Negative correlations were observed between catalase and lipid hydroperoxide (r=-0.293; p=0.018) and between myeloperoxidase and sulfhydryl groups (r=-0.267; p=0.031) in operating room personnel.
Conclusions:
- Operating room personnel exhibit elevated oxidative stress.
- This oxidative stress is potentially linked to the effects of anesthetic gas exposure.
