Complete Genome Sequences of T1-Like Phages JMPW1 and JMPW2

Area of Science:

• Microbiology and Virology
• Bacteriophage genomics
• Escherichia coli phage research

Background:

• Bacteriophages are viruses that infect bacteria, playing crucial roles in microbial ecosystems and bacterial evolution.
• T1-like phages are a group of bacteriophages known to infect Escherichia coli, a common model organism and opportunistic pathogen.
• Understanding phage genomes is essential for phage therapy development and microbial population control.

Purpose of the Study:

• To determine and analyze the complete genome sequences of two novel T1-like Escherichia coli phages, designated JMPW1 and JMPW2.
• To compare the genomic features of JMPW1 and JMPW2 with the reference phage T1.
• To identify genetic variations, particularly in genes with unknown functions and those encoding structural or enzymatic proteins.

Main Methods:

• Isolation of bacteriophages JMPW1 and JMPW2 from contaminated experimental samples.
• Whole-genome sequencing of both phages using established next-generation sequencing technologies.
• Bioinformatic analysis, including genome assembly, annotation, and comparative genomics against the T1 phage genome.

Main Results:

• Complete genome sequences were obtained for JMPW1 (49,840 bp) and JMPW2 (50,298 bp).
• Both phages exhibit high overall genomic identity with the T1 phage.
• Significant genetic differences were identified, primarily in genes of unknown function and genes encoding tail fiber proteins and endonucleases.

Conclusions:

• The genomic data provide a foundation for further functional studies of JMPW1 and JMPW2.
• The identified genetic variations may contribute to differences in host range, lytic activity, or other biological properties compared to T1.
• These findings enhance our understanding of T1-like phage diversity and evolution within the Escherichia coli species.