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Complementation of a Dictyostelium discoideum thymidylate synthase mutation with the mouse gene provides a new
A C Chang1, K L Williams, J G Williams
1School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Nucleic Acids Research
|May 25, 1989
Abstract:
A cDNA encoding mouse thymidylate synthase has been inserted 3' to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at high frequency. These transformants grew in standard axenic medium without requiring exogenous thymidine. This construct provides a second selectable marker for use in transformation of D.discoideum.