Related Experiment Video

Updated: Mar 19, 2026

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
10:43

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Published on: December 23, 2022

4.2K

Cohesin subunits, STAG1 and STAG2, and cohesin regulatory factor, PDS5b, in oral squamous cells carcinomas

Josiane Alves França1, Marina Gonçalves Diniz2, Vanessa Fátima Bernardes1

  • 1Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte, Brazil.

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
|June 25, 2016
PubMed
Abstract

Insights

Loss of heterozygosity at STAG1 and STAG2 occurs in oral squamous cell carcinoma (OSCC), but gene expression remains similar to normal tissue. PDS5B protein levels were high in OSCC but did not correlate with proliferation markers.

Area of Science:

  • Genetics and Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • The cohesin complex, including STAG1/STAG2, regulates sister chromatid cohesion and is influenced by PDS5B, a cell proliferation inhibitor.
  • Alterations in STAG1, STAG2, and PDS5B have been implicated in various tumors.
  • This study investigates these genes in oral squamous cell carcinoma (OSCC).

Purpose of the Study:

  • To assess molecular alterations of STAG1, STAG2, and PDS5B in OSCC.
  • To predict gene expression based on cell cycle gene profiles.
  • To examine the relationship between PDS5B protein expression and proliferation markers (Ki-67, p53).

Main Methods:

  • Loss of heterozygosity (LOH) analysis for STAG1 and STAG2 using polymorphic markers in 15 OSCC samples.
  • Quantitative PCR (qPCR) to compare STAG1, STAG2, and PDS5B transcript levels between OSCC and normal oral mucosa (NM).
  • Immunohistochemical analysis of PDS5B, Ki-67, and p53 in 62 OSCC samples and multiple linear regression using an 84-gene cell cycle array.

Main Results:

  • LOH was detected at STAG1 and STAG2 loci in a subset of OSCC samples.
  • Transcriptional levels of STAG1, STAG2, and PDS5B were similar between OSCC and NM.
  • High PDS5B, p53, and Ki-67 immunoexpression was observed, but PDS5B levels did not correlate with Ki-67 or p53.
  • A potential relationship was found between STAG1 and CUL3 expression.

Conclusions:

  • While LOH occurs at STAG1 and STAG2 in OSCC, their transcriptional levels are comparable to normal tissue.
  • High PDS5B protein expression in OSCC does not appear to be associated with the proliferation index.
  • Further research may explore the role of STAG1 and CUL3 interactions in OSCC.
Keywords:
PDS5BSTAG2STAG1cell cycle genesoral cancer

More Related Videos

Development of Compendium for Esophageal Squamous Cell Carcinoma03:36

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

903
A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells06:38

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Published on: January 22, 2021

7.0K

Related Concept Videos

Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.8K
Cohesins02:20

Cohesins

2.4K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.7K
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
51.4K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.3K
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
4.4K