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Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
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Effects of Human Fibroblast-Derived Extracellular Matrix on Mesenchymal Stem Cells
Yaxian Zhou1,2, Michael Zimber3, Huihua Yuan1,4
1Department of Orthopedics and Rehabilitation, Laboratory of Musculoskeletal Biology and Regenerative Medicine, University of Wisconsin-Madison, 1111 Highland Avenue, WIMR 5051, Madison, WI, 53705-2275, USA.
Stem Cell Reviews and Reports
|June 26, 2016
Summary
Bioengineered human dermal extracellular matrix (hECM) enhances human mesenchymal stem cell (hMSC) proliferation and promotes chondrogenesis, suggesting its potential for cartilage tissue engineering applications.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Tissue Engineering
Background:
- The stem cell niche, particularly the extracellular matrix (ECM), critically regulates cell behavior.
- Human mesenchymal stem cells (hMSCs) are crucial for regenerative medicine, but their behavior is microenvironment-dependent.
Purpose of the Study:
- To investigate the impact of bioengineered human dermal fibroblast-derived ECM (hECM) on hMSC proliferation and multilineage differentiation.
- To assess hECM's potential for enhancing chondrogenesis in hMSCs.
Main Methods:
- hMSCs were cultured on hECM for two passages.
- Analysis of stemness and lineage-specific markers via mRNA expression.
- Induction of osteogenesis, adipogenesis, and chondrogenesis in hMSCs cultured with or without hECM.
Main Results:
- hECM significantly increased hMSC proliferation and the proportion of cells in the S phase.
- hECM culture maintained the CD90+/CD105+/CD73+/CD45- stem cell marker profile.
- hECM downregulated osteogenesis and adipogenesis but upregulated chondrogenesis, increasing collagen type 2 production.
Conclusions:
- Bioengineered hECM promotes hMSC proliferation and enhances chondrogenesis.
- hECM shows promise as a biomaterial for culturing hMSCs for cartilage tissue engineering.
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