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Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local
J F Schwensen1, C Menné Bonefeld2, C Zachariae3
1National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark.
The British Journal of Dermatology
|June 26, 2016
Summary
High rates of contact allergy to methylisothiazolinone (MI) prompted a study on cross-reactivity. Data indicate cross-reactivity between MI, octylisothiazolinone (OIT), and benzisothiazolinone (BIT) when chemical potency is considered.
Area of Science:
- Dermatology
- Toxicology
- Immunology
Background:
- High rates of contact allergy to methylisothiazolinone (MI) necessitate understanding cross-reactivity.
- Investigating cross-reactivity between MI, octylisothiazolinone (OIT), and benzisothiazolinone (BIT) is crucial.
Purpose of the Study:
- To investigate the cross-reactivity between methylisothiazolinone (MI) and octylisothiazolinone (OIT).
- To investigate the cross-reactivity between methylisothiazolinone (MI) and benzisothiazolinone (BIT).
Main Methods:
- A modified local lymph node assay was used in female CBA mice.
- Immune responses were assessed by measuring ear thickness and T and B cell proliferation.
- Sensitization with MI was followed by challenges with MI, OIT, and BIT at equipotent concentrations.
Main Results:
- Methylisothiazolinone (MI) induced significant, concentration-dependent immune responses.
- No significant differences in T cell proliferation were observed between MI, OIT, and BIT challenges.
- The study used concentrations corresponding to individual EC3 values for challenge.
Conclusions:
- Cross-reactivity exists between MI, OIT, and BIT when chemical potency is considered.
- Individuals sensitized to MI may exhibit reactions to OIT and BIT upon sufficient exposure.
- Findings highlight the importance of considering cross-reactivity in preservative allergy management.
