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A clinical Acanthamoeba isolate harboring two distinct bacterial endosymbionts
Anneliese Müller1, Julia Walochnik2, Martin Wagner1
1Institute for Milk Hygiene, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
European Journal of Protistology
|June 27, 2016
Summary
This study details the stable co-occurrence of two bacterial symbionts, Parachlamydia and Paracaedibacter, within Acanthamoeba hatchetti. These findings highlight complex microbial interactions within Acanthamoeba hosts.
Area of Science:
- Microbiology
- Cell Biology
- Parasitology
Background:
- Acanthamoebae are protozoa known to host bacterial symbionts.
- The co-occurrence of multiple symbionts within a single Acanthamoeba host is increasingly recognized.
Purpose of the Study:
- To describe the stable co-occurrence of two distinct bacterial symbionts within a clinical isolate of Acanthamoeba hatchetti.
- To characterize the nature of this dual symbiosis using advanced microscopy techniques.
Main Methods:
- Fluorescence in situ hybridization (FISH) was used to identify and localize the symbionts.
- Transmission electron microscopy (TEM) provided ultrastructural details of the symbionts within the Acanthamoeba host.
Main Results:
- A stable co-occurrence of a Parachlamydia-affiliated bacterium and a Paracaedibacter (Alphaproteobacteria) bacterium was observed within Acanthamoeba hatchetti (genotype T4).
- FISH and TEM confirmed the presence and intracellular location of both symbionts within the same host cell.
Conclusions:
- This study provides evidence for the simultaneous co-habitation of two different bacterial symbionts within a single Acanthamoeba cell.
- The findings contribute to the understanding of host-symbiont dynamics and the diversity of microbial communities associated with Acanthamoeba.
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