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Updated: Mar 18, 2026
A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
Published on: May 2, 2018
Spiral-based microfluidic device for long-term time course imaging of Neurospora crassa with single nucleus
Kang Kug Lee1, Laszlo Labiscsak2, Chong H Ahn3
1Computational and Molecular Biology Laboratory, Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267, USA; Microsystems and BioMEMS Laboratory, Department of Electrical Engineering and Computing Systems, University of Cincinnati, Cincinnati, OH 45221, USA.
Abstract:
Real-time imaging of fluorescent reporters plays a critical role in elucidating fundamental molecular mechanisms including circadian rhythms in the model filamentous fungus, Neurospora crassa. However, monitoring N. crassa for an extended period of time with single nucleus resolution is a technically challenging task due to hyphal growth that rapidly moves beyond a region of interest during microscopy experiments. In this report, we have proposed a two-dimensional spiral-based microfluidic platform and applied for monitoring the single-nucleus dynamics in N. crassa for long-term time course experiments.
