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Interactions between the Coxiella burnetii parasitophorous vacuole and the endoplasmic reticulum involve the host

Anna V Justis1, Bryan Hansen2, Paul A Beare3

  • 1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.

Cellular Microbiology
|June 28, 2016
PubMed

Insights

Coxiella burnetii infection hijacks host cell processes by recruiting oxysterol-binding protein-related protein 1 long (ORP1L) to its vacuole. This protein is crucial for maintaining the vacuole

Area of Science:

  • Microbiology
  • Cell Biology
  • Host-Pathogen Interactions

Background:

  • Coxiella burnetii, an intracellular bacterium, requires a specialized vacuole for replication.
  • Host cell lipids are vital for this vacuole, but Coxiella's manipulation mechanisms are unclear.
  • Oxysterol-binding protein-related protein 1 long (ORP1L) impacts endocytic trafficking and ER-endosome interactions.

Purpose of the Study:

  • To investigate the role of ORP1L in Coxiella burnetii infection.
  • To determine how ORP1L interacts with the Coxiella parasitophorous vacuole (PV).

Main Methods:

  • Tracking ORP1L localization during infection using microscopy.
  • Employing the Coxiella Dot/Icm secretion system for effector protein delivery.
  • Utilizing electron microscopy to visualize membrane contacts.
  • Depleting ORP1L to assess its impact on PV size.

Main Results:

  • ORP1L rapidly localized to the Coxiella PV via the Dot/Icm system.
  • ORP1L's ankyrin repeats mediated PV binding, suggesting interaction with a PV protein.
  • ORP1L co-localized with the ER at the PV, forming membrane contact sites.
  • ORP1L depletion resulted in significantly smaller PVs.

Conclusions:

  • ORP1L is recruited to the Coxiella PV by the bacterium.
  • ORP1L influences PV membrane dynamics and ER interactions.
  • ORP1L plays a key role in maintaining the intracellular niche for Coxiella replication.

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